FIG. 5.
Application of PCR with TFR primers to diagnostic samples. (A) Analysis of products by 10% acrylamide gel electrophoresis. (B) Detection of products by DEIA with biotinylated oligonucleotide TFR8-Bio. Lanes 2 to 8, diagnostic samples from seven different bulls with suspected T. foetus infection; lane 1, T. foetus-positive control; lane 9, negative control. The absorbance units (AU) presented in panel B are A630 values subtracted from A450 values. Animals were retested at different times; results for only one sample per bull are shown.