TABLE 5.
Published PCR conditions for primers used in this studya
| Source | Fragment (size [bp]) | Concn of each dNTP | Amt of Taq (U) | Vol | No. of PCR cycles | Cycles | pH | MgCl2 concn (mM) |
|---|---|---|---|---|---|---|---|---|
| Perkin-Elmer Cetus | 200 mM | 2.5 | 100 μl | 8.3 | 1.5 | |||
| Spector and Wolf (17) | EcoRI-D (152) | 200 mM | 2.5 | 100 μl | 35 | 94°C for 1 min, 55°C for 2 min, and 72°C for 3 min | 8.3 | 5 |
| Drouet et al. (3) | HindIII-X (406) | 200 μM | 2.5 | 100 ml | 30 | 95°C for 30 s, 55°C for 1 min, and 72°C for 1 min | 8.4 | 1.5 |
| Espy et al. (4) | MIE gene (370) | 200 μM | 1.25 | 50 ml | 60 | 94°C for 1 min, 60°C for 1 min, and 72°C for 1 min | 8.3 | 1.5 |
a
For all sources, PCR buffer and 10 mM Tris-HCl were used. dNTP, deoxynucleoside triphosphate.