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. 2023 Apr 7;30(7-8):649–658. doi: 10.1038/s41434-023-00399-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Experimental setup, showing placement of stimulation (S1 and S2) and recording electrodes (Rec.) in the stratum radiatum, CA1 area of the acute hippocampal slice (left) and schematic depiction of the stimulation protocol (right). The effect of transgene NPY expression on excitatory neurotransmission was also examined by applying repetitive train stimulation (TS) with 100 Hz, 10 stimulations, in one group of presynaptic fibers/pathway (S1), preceded and followed at 500 ms interval by PP-stimulation (20 Hz) in a neighbouring, convergent but independent presynaptic pathway (S2). We compared the amplitude of the fEPSPS evoked by paired-pulse stimulation (PP) before (PP1) and after train stimulation (PP2, blue B). Example sweeps showing fEPSP evoked by S2 before (black sweep) and after TS-stimulation (blue sweep), showing a reduction in the first fEPSP and concomitant increase in PPR after TS-stimulation. Right) This reduction is smaller in the AAV1-NPY/Y2 treated animals after application of the Y2-receptor antagonist BIIE0246. C Bar graph showing average depression of fEPSP after TS-stimulation in AAV1-NPY/Y2 (n = 5), AAV1-Y2/NPY (n = 7), and AAV1-EMPTY (n = 7). The TS-induced depression of fEPSP is reduced after application of BIIE0246 in the AAV1-NPY/Y2 treatment-group (n = 5), but not in AAV1-Y2/NPY (n = 7) or AAV1-EMPTY (n = 7). AAV1-NPY/Y2 (left bars), AAV1-Y2/NPY (middle bars), and AAV1-EMPTY (right bars). D Examples of immunohistochemical staining for NPY after electrophysiology. Scalebar 100 μm, **P < 0.01, ***P < 0.001, Student’s paired t-test. Data is shown as mean ± SEM.