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. 2023 Aug 25;14:5208. doi: 10.1038/s41467-023-40981-9

Fig. 4. PAF1 and SSRP1 are required for the activity of MLL-AF4-bound and non-MLL-AF4-bound enhancers.

Fig. 4

a Western blot for PAF1 or SSRP1 in wild-type (WT), PAF1 degron or SSRP1 degron cells, with (+) or without (−) addition of 0.5 µM dTAG-13 for 24 h. Blots are representative of three replicates. Bands representing wild-type and FKBP12 F36V-tagged proteins are indicated. Source data are provided as a Source Data file. b Mean distribution of strand-specific TT-seq (eRNA) levels at MLL-AF4-bound and non-MLL-AF4-bound intergenic enhancers in PAF1 degron or SSRP1 degron cell lines under control (untreated) and 24 h dTAG-13-treated conditions. Lines represent mean, shading represents ± SEM, n = 3 independent experiments. c TT-seq and reference-normalized ChIP-seq/TOPmentation at the FLT3 locus in PAF1 degron and SSRP1 degron SEM cells, with or without the addition of dTAG-13 for 24 h. d Mean distribution of H3K27ac, MLL and H3K79me3 at MLL-AF4-bound and non-MLL-AF4-bound enhancers in PAF1 degron (above) or SSRP1 degron (below) cell lines under control (untreated) and 24 h dTAG-13-treated conditions.