Fig. 5. MLL-AF4 binding is necessary to maintain enhancer-promoter interactions.

a Capture-C from the promoters of ARID1B, CDK6, and FLT3 in SEM cells under control (purple) and 96 h MLL-AF4 knockdown (green) conditions (upper) or in PAF1 degron or SSRP1 degron cell lines under control (purple) and 24 h dTAG-13-treated (green) conditions. Lines represent mean, shading represents ± SEM, n = 3 independent experiments. ChIP-seq traces for MLL, AF4, PAF1 and SSRP1 are shown, along with bioinformatically-annotated MLL-AF4-bound (purple bars) and -unbound (gray bars) enhancers. b Statistical analysis of the changes in Capture-C interaction frequency between promoters and MLL-AF4-bound or -unbound enhancers following the indicated treatments. Size and color of the dot are proportional to the significance of the change in interaction with each enhancer. n = 3; ns: adjusted p-value ≥ 0.05; two-sided Mann–Whitney U test. The 7-day DOT1Li data²⁸ and 24-h BRD4 PROTAC (AT1) data²⁶ were previously published. c Correlation of changes in Capture-C interaction frequency between promoters and MLL-AF4-bound (upper triangle) or -unbound (lower triangle) enhancers, comparing the indicated treatments.