Figure 6.

Suppression of integrin αv and β3 cell surface expression by the treatment of LAT1 inhibitors. (A) Cell surface expression of integrin αv and integrin β3. Cell surface expression of integrin αv and integrin β3 in B16-F10 cells treated with nanvuranlat (3, 10, and 30 μM) or BCH (20 mM) for 24 h was detected by flow cytometry. Data are represented as histograms showing fluorescence intensity (y-axis) versus cell count (x-axis). Gray-filled peaks represent unstained B16-F10 cells (without inhibitor treatment), red open peaks represent cells without inhibitor treatment, and colored peaks indicate cells treated with the inhibitors. (B, C) Quantitative analysis of MFI (Median Fluorescence Intensity, relative to cells without inhibitor treatment) of B16-F10 cells treated with nanvuranlat or BCH. Statistical significance was determined using one-way ANOVA followed by Tukey's post-test (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). Data are mean ± SEM.