TABLE 1.
Components and interpretation of the RapID CB Plus panel tests
Test or substrate | Positive reaction | Negative reaction |
---|---|---|
Carbohydrate utilization tests | ||
Glu (glucose) | Yellow, gold, or light yellow-orange | Red, dark orange, or orange |
Suc (sucrose) | ||
Rib (ribose) | ||
Mal (maltose) | ||
Substrates for glycosidase tests | ||
α-GLU (p-nitrophenyl-α-d-glucoside) | Yellow | Clear, tan, or very pale yellow |
β-GLU (p-nitrophenyl-β-d-glucoside) | ||
NAG (p-nitrophenyl-N-acetyl-β-d-glucosaminide) | ||
GLY1 (p-nitrophenylglycoside) | ||
ONPG (o-nitrophenyl-β-d-galactopyranoside) | ||
Substrates for aminopeptidases | ||
PRO (proline-β-naphthylamide) | ||
TRY (tryptophan-β-naphthylamide) | ||
PYR (pyrrolidine-β-naphthylamine) | Purple, red, or very dark purple | Yellow, light orange, or very light pink |
LGLY (leucyl-glycine-β-naphthylamide) | ||
LEU (leucine-β-naphthylamide) | ||
Single-enzyme tests | ||
URE (urease) | Red or dark red-orange | Yellow or orange |
NIT (nitrate reductase) | Red or pink | Clear |
PHSa (phosphatase) | Yellow | Clear, tan, or very pale yellow |
LIP (lipase) | Yellow or yellow-orange | Red or orange |
PHS is also a p-nitrophenyl test.