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. 2023 Aug 9;9(8):e18920. doi: 10.1016/j.heliyon.2023.e18920

Table 7.

Chemically and electrically induced seizure studies in rats with PO and IP administration of cenobamate, YKP3090, and YKP1983 (NINDS study, n = 8–24/group).

Seizure Model Parameter PO
IP
Cenobamate YKP3090 YKP1983 Cenobamate YKP3090 YKP1983
Minimum motor impairment TD50 mg/kg (95% CI) 50.7 (35.7–63.0) <150 81.1 (61.1–112) 38.9 (32.2–43.9) 50.9 (33.1–67.8) 53.3 (41.5–62.2)
SC PTZ 56.4 mg/kg or 68 mg/kga ED50 mg/kg (95% CI) Max. 40% protection at 25 8.14 (0.74–16.9) Max. 33% protection at 12.5 13.6 (6.6–25.0) 16.7 (14.8–18.7) 19.3 (12.4–28.5)
PIb NA <18 NA 2.9 3.0 2.8
Hippocampal kindling ED50 mg/kg (95% CI) NT NT NT 16.4 (12.7–20.2) >50 (−) Inactive at 30
PIb NA NA NA 2.4 <1 NA
MES ED50 mg/kg (95% CI) 1.9 (0.9–3.3) 11.9 (8.5–15.0) 2.9 (2.0–3.7) 2.9 (1.9–3.8) 26.1 (14.6–41.9) 8.4 (6.9–9.8)
PIb 27 <13 28 14 2.0 6.4

CI, confidence interval; ED50, median effective dose; IP, intraperitoneal; MES, maximal electroshock seizures; NA, not applicable/available; NINDS, National Institute of Neurological Disorders and Stroke; NT, not tested; PI, protective index; PO, oral; PTZ, pentylenetetrazol; SC, subcutaneous; TD50, median neurotoxic dose.

a56.4 mg/kg for rats sourced from Simonsen Laboratories, Inc. and 68 mg/kg for rats sourced from Charles River Laboratories.

bProtective index calculation TD50/ED50. TD50 based on minimal motor impairment.