Figure 2.

Normal and NTDs mouse fetal brain metabolome

(A) Experimental group and time lines. Low-folate diets combined with MTX-induced NTDs (Spina Bifida) in C57BL/6 mouse embryos.

(B) H&E staining of brain tissue pathological changes of mice.

(C and D) PCA analysis to the overall distribution and stability of each sample, and spolt-OPLS-DA to distinguish the global metabolic differences between groups and identify the differential metabolites between NTDs and Con group (n = 6 per group).

(E) The violin maps of each sample in the two groups.

(F) The bubble diagram of KEGG pathway enrichment in the two groups.