LC-MS of fecal in pregnant mice
(A and B) PCA analysis to the overall distribution and stability of each sample, and spolt-OPLS-DA to distinguish the global metabolic differences between groups and identify the differential metabolites between NTDs and Con group(n = 6 per group).
(C) Cluster heatmap of differential metabolites (p < 0.05).
(D) Volcano plots of all differential metabolites between two groups, the p values are described in Table S4.
(E) Representative differential metabolites in the two group were screened out, the KEGG terms shown on the right side (n = 6, the p values are described in Table S5).