REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
The human neural tube defects fetal brain tissues	Lvliang area of Shanxi Province in northern China	N/A
Healthy fetal brain tissues	Lvliang area of Shanxi Province in northern China	N/A
Chemicals, peptides, and recombinant proteins
Methotrexate	Sigma	Cat#06563
Critical commercial assays
TruSeq Nano DNA LT Sample Prepararion Kit	Illumina	Cat#FC-121-4001
KAPA Library Quantification Kits	Kapa Biosystems	Cat#KK4824
Deposited data
Metagenomics data	NCBI	BioProject PRJNA896594
Mass spectrometry data	Metabolights	MTBLS4893 and MTBLS4894
Experimental models: Organisms/strains
C57BL/6 mice	Charles River Laboratories (CRL)	Cat#213
Software and algorithms
Prism 9.0	Graphpad	https://www.graphpad.com/scientific-software/prism
R	The R Foundation	https://www.r-project.org/about.html
R Studio	R Studio	https://rstudio.com/
SPSS	IBM SPSS Statistics	https://www.ibm.com/spss
Other
Low folate diet chow	Beijing Keao Xieli Feed Co.,Ltd	N/A