Figure 2.

Sorting performance of the microfluidic chip sorter

(A) The recovery at various interval times “t” was confirmed by applying a fake acquisition operation prior to the true operation, using 5 μm polystyrene particles. (B) The viability, purity, and effective yield at various event rates with two different target populations using PBMCs. Solid circles show viability, solid rectangles show purity, and solid triangles show effective yield. n = 3, 3, and 13 at 7,000, 10,000, and 15,000 events per second, respectively, at the target population of 35.9% (sort gate: lymphocyte+CD8+). n = 3, 3, and 7 at 7,000, 10,000, and 15,000 events per second, respectively, at the target population of 52.7% (sort gate: lymphocyte+CD4+). Error bars represent standard deviation of the mean. (C) The viability, purity, and effective yield at various target populations with three different event rates using PBMCs (n > 3). Error bars represent standard deviation of the mean. (D) Representative flow cytometry data before and after sorting at three different event rates, used two different target populations.