Figure 3.

Viability and cell proliferation analysis using NY-ESO-1 TCR-T cells

(A) The average viability is shown after sorting using the microfluidic sorter (n = 12) and droplet sorter (n = 6) at event rates from 1,000 to 1,300 events per second. Error bars represent standard deviation of the mean. (B) Representative flow cytometry data after sorting lymphocyte+CD8+Tet+ (tetramer+) TCR-T cells using the microfluidic sorter and droplet sorter. (C) The result of triplicate WST-1 assays on days 0, 1, 2, and 3 after sorting. Solid rectangles show the result for non-sorted (control) cells, solid circles show the results obtained using the microfluidic chip sorter, and solid triangles show the results obtained using the droplet sorter. Error bars represent standard deviation of the mean.