Figure 4.
Cytokine secretion and cytotoxicity analysis using NY-ESO-1 TCR-T cells
(A) IFN-γ spots were counted to show the potency of the cytokine produced by sorted TCR-T cells as determined by performing the ELISPOT assay in triplicate. Error bars represent standard deviation of the mean. The IFN-γ spot counts of all TCR-T cells from donor #3 treated with ESO1-T2 were saturated (A, right), and thus the number of counts was set to 500. (B) Cytotoxic activity of sorted TCR-T cells was shown by performing the 51Cr-release assay in triplicate. Error bars represent standard deviation of the mean. Solid rectangles show the results following reaction with ESO1-T2 (T2 cells pulsed with NY-ESO-1p157-165), solid circles show the results following reaction with MAGEA4-T2 (T2 cells pulsed with MAGE-A4p230-239 9), solid triangles show the results following reaction with NW-MEL-38, and solid diamonds show the results following reaction with SK-MEL-52.