Table 1.

The summary of our technology in comparison to droplet sorter

	Droplet sorter (traditional cell sorter)	Microfluidic chip sorter (our system)
Optics	laser excitation, forward- and side-scatter detection, fluorescence detection (up to 13–18 markers) ∗BD FACSAria I ∼ III	laser excitations, forward- and back-scatter detection, fluorescence detection (up to 8 markers)
Fluidics	hydrodynamic focusing for detection	hydrodynamic focusing for detection
	open system to generate droplets in air (jet-in-air)	closed system to acquire cells of interest in microfluidic chip
	cleaning is required for fluidic parts	all fluidic parts are single-use disposable
	inline filter is not normally implemented	inline filter is implemented
Others	–	clog detection and recovery technology