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. 2023 Aug 26;12(9):12353. doi: 10.1002/jev2.12353

FIGURE 2.

FIGURE 2

Flow chart of the procedure used to obtain EV‐enriched fractions using differential centrifugation and ultracentrifugation. Following cell culture preparation, the culture supernatant was collected and underwent a set of centrifugation steps at progressively higher spin speeds. First, large particles including dead cells, cell debris, and apoptotic bodies were removed using two differential centrifugation steps (2,000 g and 10,000 g). Then, EV‐enriched fractions were separated from smaller contaminants such as proteins, using ultracentrifugation (100,000 g).