TABLE 2.
Comparison of different (experimental and theoretical) methods to determine electrical valence of BSA protein (pH of 8.1, 25°C).
| Electrical valence from theoretical/experimental technique | Determined value | Ref. |
|---|---|---|
| Effective valence (Zeff ) | ‐4.08 | This study |
| Debye‐Hückel‐Henry valence (ZDHH ) | ‐25.86 | This study |
| Calculated electrical valence using Ohshima's expression (ZOhshima ) | ‐17.99 | This study |
| Computed electrical valence from amino acid composition using PROPKa3 | ‐16.00 | Olsson et al., 2011; Filoti et al., 2015 |
| Computed electrical valence from amino acid composition using Sednterp | ‐18.30 | Filoti et al., 2015 |
| Electrical valence using hydrogen ion titration | ‐17.00 | Tanford et al., 1955 |
Note: The measured EM of BSA was −1.12 (μm.cm.V−1.s−1) assuming a radius of 3.48 nm. (Filoti et al., 2015).