Table 2.

Sample preparation details for each analytical technique used in this study

Technique	Amount of lipids	Solvent	Spraying Area	Sample Support	Hydration
Permeability	1 mg	hexane/ ethanol (2:1, v/v)	1 × 1 cm2	nucleopore polycarbonate disk	1 h, PBS in diffusion cell, at 32°C
TEWL	1 mg	hexane/ ethanol (2:1, v/v)	1 × 1 cm2	nucleopore polycarbonate disk	30 min, water in diffusion cell, room temperature
SAXD	1 mg	hexane/ ethanol (2:1, v/v)	2 × 3 mm2	nucleopore polycarbonate disk	24 h, 80% relative humidity, room temperature
FTIR	1 mg	chloroform/ methanol (2:1, v/v)	1 × 1 cm2	silver bromide (AgBr) window	≥12 h, acetate buffer (pH 5) in D2O, at 37°C
Neutron diffraction	10 mg	chloroform/ methanol (2:1, v/v)	1.2 × 3.8 cm2	silicon substrate	≥12 h, D2O/H2O buffer (100%, 50%, 8%), at 37°C