Fig. 5.

Microbial depletion abolishes the anti-obesogenic effects of PCRCP in mice fed with HFD. (A) Diagram of antibiotics (vancomycin and neomycin) treatment of faecal bacteria. Animals were pretreated for one week with a cocktail of antibiotics added to the drinking water and provided orally with water or PCRCP for four weeks. (B) The initial and final body weight of mice. (C) The daily food intake of mice. (D-F) Visceral fat pad weight of gWAT, iWAT and pWAT. (G-H) Glucose tolerance and its area under curve. (I-M) The plasma levels of TG, CHO, LDL-C, ALT and AST. Data present the mean ± s.e.m. (n = 5–7). Statistical analysis was carried out by using Student’s t-test, and ns means no statistical significance.