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. 2023 Aug 16;34(15-16):682–696. doi: 10.1089/hum.2022.211

Figure 2.

Figure 2.

Pericytes are efficiently transduced by AAV-PR after systemic injection. The identity of transduced vascular cells in Ai9 mice injected with AAV-PR-CBA-Cre was determined by immunofluorescence staining. (A) Low magnification image α-SMA staining (yellow) for pericytes and tdTomato (purple). Scale bar = 100 μm. Inset: higher magnification of the cortex showing colocalization. Scale bar = 50 μm (B, C). Higher magnification images of tdTomato/α-SMA colocalization. Scale bar = 10 μm (D). Colocalization of tdTomato with PDGFR-β. Bottom panel shows PDGFR (yellow), tdTomato (purple), DAPI (cyan), and CD31 endothelial cells (white). The colocalization of tdTomato was mainly with PDGFR and not CD31. (B–D) Arrows head point to transduced pericytes. (E) Quantitation of transduction efficiency in pericytes (PDGFR) and endothelial cells (CD31). Colocalization was based on a Pearson correlation of >0.35. (F) Transduction of smooth muscle cells on large caliber vessels. Arrow points to transduced smooth muscle cells. α-SMA, α-smooth muscle actin; DAPI, 4′,6-diamidino-2-phenylindole; PDGFR, platelet-derived growth factor receptor.