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. 2023 Aug 16;34(15-16):705–718. doi: 10.1089/hum.2023.016

Figure 4.

Figure 4.

S10 shuttle delivery of DRI-NLS-Cy5 peptide to ferret airway epithelia in vivo. (A) Schematic showing the experimental workflow for the S10-mediated delivery of Alexa Fluor Cy5 conjugated DRI-NLS (DRI-NLS-Cy5) peptide into ferret airways. The DRI-NLS-Cy5 peptide (10 μM) was delivered with S10 (20 μM) intratracheally to wild-type ferret airways at a dose of 1.0 mL/kg body weight. The delivery efficiency was evaluated at 30 min postdelivery. (B) Representative fluorescent images of tissue sections showing DRI-NLS peptide (magenta) in adult (top panel) and kit (bottom) ferret tracheal epithelia. (C, D) Representative immunofluorescent image colocalization of DRI-NLS-Cy5 peptide (gray) with cellular markers for ciliated cells (α-tubulin, red) and goblet cells (MUC5AC, green) in ILB airway epithelia of adult (C, ci) and kit (D) ferret lungs. (ci) Inset shows magnification of the red boxed area in (C). (E, F) The efficiency of S10/DRI-NLS peptide delivery to adult (E) and kit (F) TR. (G, H) The frequencies of S10/DRI-NLS peptide delivery to the indicated epithelial cell types in adult (G) and kit (H) TR. Bars equal 100 μm (B–D) and 20 μm (inset ci). Data in (E–H) represent the mean ± SD (N = 26–34 randomly quantified fields, from three ferrets per group). Individual count areas are represented by distinct data points in bars of each graph.