Figure 5.

Proportion of splenic CD8⁺ T cells measured by intracellular cytokine staining. (A) The count of CD3⁺, CD4⁺, and CD8⁺ T lymphocytes in the spleen was quantified by flow cytometry. Representative flow cytometric dot plot demonstrates the gating strategy employed for analyzing subsets of T lymphocytes based on forward and sidelight scatter. Dot plots exhibiting double-positive staining indicate the CD8⁺ T cells (Q-UR). (B) The CD8⁺ T cells rates in the spleen, shown as a percentage, were assessed by flow cytometry. * p < 0.05, and *** p < 0.001 indicate statistically significant differences among various groups.