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. 2023 Aug 3;12(8):1012. doi: 10.3390/pathogens12081012

Figure 2.

Figure 2

Effect of BTU-1 (26.4 µM), BTU-2 (13.4 µM), and BTU-3 (61.1 µM) on the growth, metabolic activity, and volume of epimastigotes of Trypanosoma cruzi Y strain. (a) Growth kinetics of epimastigotes in the presence of BTUs for 96 h were evaluated by directly counting the number of epimastigotes every 24 h. (b) The metabolic activity of epimastigotes was determined using resazurin dye every 24 h. (c) Cell volume was determined by flow cytometry after incubation in the presence of BTUs for 24 h. The data represent the mean ± standard deviation of three independent experiments. Actinomycin D was used as a positive control for cell volume analysis. * p < 0.05; ** p < 0.01; and **** p < 0.0001 compared to untreated parasites (control).