TABLE 2.
Nucleotide sequences of codons of isolates from patient specimens which were indeterminant by OLA, including 10 bases on either side of the ligation site
| Patient sample no. | Results of direct sequencing (5′→3′)a | pol amino acid |
|---|---|---|
| 1 | AC-AGT-GAT-AA*A-TGG-AGA-AAA | K70 |
| 2 | TGT-ACA-GAG-A*TG-GAA-AAG-GA | M41 |
| 2 | AC-AGT-GAC-AG*A-TGG-AGA-AAA | K70R |
| 2 | TGG-AGA-AAA-A*TA-GTA-GAT-TT | L74I |
| 3 | AC-AGT-ACT-GG*A-TGG-AGA-AAA | K70G |
| 4 | GG-GGA-TTT-TG*C-ACA-CCA-GAC | T215C |
| 5 | TGT-ACA-GAG-A*TG-GAA-AAG-GA | M41 |
| 6 | AC-AGT-ACT-AA*G-TGG-AGA-AAA | K70 |
| 7 | TGG-AGA-AAG-T*TA-GTA-GAT-TT | L74 |
| 8 | TGT-ACA-GAT-A/C*TG-GAA-AAG-GA | M41M/L |
| 8 | GG-GGA-TTT-AC*T-ACA-CCA-GA | T215 |
| 9 | TGT-ACA-GAG-A*TG-GAA-AAG-GA | M41 |
| 10 | TGG-AGA-AAA-A*TA-GTA-GAT-TT | L741 |
| 11 | TGT-ACA-GAT-A*TG-GAA-AAG-GA | M41 |
| 11 | GG-GGA-TTT-A/GT*T-ACA-CCA-GA | T215F/L |
a
Boldface type indicates base changes which were not complementary to the ligation oligonucleotides. *, ligation site. The codon of interest is underlined.