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. 2023 Aug 9;11(8):2040. doi: 10.3390/microorganisms11082040

Figure 1.

Figure 1

Inactivation of ETEC by Psoralen + UVA (PUVA). AMT psoralen at the indicated concentrations was added to 5 h CFA broth cultures for 1 h followed by irradiation with UVA light at (a). 1 J/cm2 and (b). 2 J/cm2; (c). AMT psoralen at 50 μg/mL was added to cultures followed by irradiation at 1, 2, and 4 J/cm2. Results depict CFU/mL remaining (mean ± s.d.) from 2–3 experiments/dose; L.O.D = limit of detection; (d). Reproducibility of killing method; independent experiments in which 1.0 mL stationary phase ETEC cultures were treated with 50 µg/mL AMT and 1 J/cm2 (n = 6) or 2 J/cm2 UVA (n = 6); 0 CFU is plotted on the x axis. Inactivation controls: heat (n = 4), formalin (n = 4); mean CFU/mL ± s.d. is shown.