Figure 2.

PUVA-ETEC are KBMA. (a). MTS assay for metabolic activity. Bacteria treated with 50 µg/mL AMT + 2 J/cm² UVA were washed and added to 96-well cultures with MTS reagent for 1 h. Bars depict the mean OD490 ± s.d. from multiple independent cultures (dots). (b). MTS assay on frozen and thawed bacteria, (n = 3 per sample); (c). KBMA activity over time. Live, PUVA-ETEC, and formalin-ETEC were added to fresh 96-well cultures. Inactivated bacterial densities were adjusted to the 24 h OD600 of live bacteria to normalize for their inability to replicate over 24 h. At 1 h and 23 h after initiation of cultures, MTS reagent was added for an additional 1 h and the absorbance at OD490 nm was measured; the mean OD490 ± s.d. from 3 cultures is shown.