Figure 4.

ETEC whole cell ELISA and immunogenicity of high dose PUVA–ETEC vaccine in mice. Nunc Maxisorp wells were coated with live H10407 ETEC (OD₆₀₀ = 0.5) suspended in PBS; no antigen control wells received PBS; (a). ETEC-coated or PBS control wells probed with rabbit anti-E.coli antiserum; (b). ETEC-coated wells probed with mouse anti-LPS antibody; (c). serum antibody response (IgM+G+A) of BALB/c mice primed and boosted with 1 × 10⁸ CFU equivalents of PUVA–ETEC (n = 5) or PUVA–ETEC + dmLT (n = 4). Pooled sera collected on day 0 (prebleed) and day 35 (2 weeks post-boost) were analyzed. Mean OD₄₅₀ ± s.d. of triplicate pools is shown in each panel. One of five mice in the PUVA–ETEC + dmLT group (panel (c)) was sacrificed before the end of the experiment due to excessive weight loss.