Table 2.

Intra- and inter-assay variability analysis of the real-time PCR assay.

Plasmid	Concentration (Copies/µL)	Intra-Assay		Inter-Assay
		$\mathbf{Mean} (\overline{\mathbf{X}}\pm \mathrm{SD})$	CV (%)	$\mathbf{Mean} (\overline{\mathbf{X}}\pm \mathrm{SD})$	CV (%)
PSV-P	4.67 × 102	31.53 ± 0.15	0.47	31.26 ± 0.26	0.83
	4.67 ×104	25.23 ± 0.03	0.14	25.10 ± 0.19	0.76
	4.67 × 106	19.41 ± 0.07	0.36	19.47 ± 0.15	0.78