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. 2023 Aug 10;15(16):3527. doi: 10.3390/nu15163527

Figure 1.

Figure 1

Ancillary protein CD147 enhances the plasma membrane localization of MCT13. (A) Effect of CD147 and GP70 on the intracellular localization of MCT13 in HEK239T cells. HEK293T cells were transfected with plasmids of EGFP-tagged MCT13 (green) and CD147 or GP70. The plasma membrane of cells was stained by PlasMem Bright Red (red). (B) Bi-FC assay for interactions between MCT13 and CD147. VN and VC mean the N- and C-terminal fragments of Venus fluorescent protein, respectively. HE293T cells were transfected with MCT13-VC and CD147-VN or -VC. EGFP-tagged MCT13 was used as a positive control. Venus fluorescent signals were detected by fluorescence microscopy. The obtained fluorescent images were merged with phase contrast images.