Figure 3.

Phylogenetic analysis of the partial typed 340 bp (with G2SKF/R) capsid region of HNoV nucleotide sequence circulating in Vhembe District (South Africa) in 2019–2021. Phylogenetic tree was set using neighbour joining method. Squared black dots indicate HNoV genotyped in this study, and reference sequences were randomly selected from GenBank, with their respective accession numbers based on high similarity with our study sequences. All positions containing gaps and missing data were eliminated. The evolutionary distances were computed using the p-distance method and are in the units of the number of base differences per site. Evolutionary analyses were conducted in MEGA 11 (10.0.5) and bootstrap tests (1000 replicates) based on the Kimura two-parameter model. Only bootstrap values greater than 70% are shown.