Figure 4.
Levels of viral and proinflammatory cytokine RNA were assessed from EVs secreted from HIV-1-infected cells treated with HU308. U1 cells (1 × 106 cells/mL) were treated with or without HU308 (5 µM) for 3 days. EV subpopulations were collected after differential ultracentrifugation spun at varying g × force, namely 2K, 10K, 100K, 167K(S), 167K(L). EV subpopulations were assessed for viral RNA TAR (A), env (B), and proinflammatory cytokine mRNA TNF-α (C) and IL-1β (D). Student’s t-test compared treated subpopulations to untreated. Red boxes indicate subpopulations of interest. */#, p < 0.05; **/##, p < 0.01; ***/###, p < 0.001; * depicts a significant decrease and # a significant increase compared to untreated. Error bars, S.D.