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. 2023 Aug 18;15(16):3638. doi: 10.3390/nu15163638

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of CR on the population of tumor-infiltrating lymphocytes (TILs). (A) Representative dot plots depicting (upper panels) or percentages (lower panel) of TILs expressing CD3 and CD45. B16-OVA-inoculated mice were treated with/without anti-PD-1 antibody (0.2 mg/mouse i.p.) at days 3, 6, and 9 from day 0. (B) Representative dot plots depicting (upper panels) or percentages (lower panel) of cells expressing CD4 and CD8 in the gated CD3⁺CD45⁺ TILs. (C) Representative histograms representing surface expression (upper panels) or median fluorescence intensity (MFI) values (lower panel) of CD44 on CD3⁺CD8⁺CD45⁺ TILs derived from B16-OVA-inoculated mice. Each plot represents the mean ± SEM (n = 4); indicated p values were obtained from a statistical comparison; one-way ANOVA with Bonferroni’s multiple comparisons correction.