Table 2.

The analysis of response variables obtained by Design-Expert Software.

Source	VS		EE		ZP		CR
	F	p	F	p	F	p	F	p
Model	290.17	< 0.0001	145.57	< 0.0001	40.09	< 0.0001	138.46	< 0.0001
X1: Span 60	7.22	0.0211	102.10	< 0.0001	0.2795	0.6067	42.62	< 0.0001
X2: Chitosan	72.38	< 0.0001	6.28	0.0292	117.86	< 0.0001	0.2698	0.6129
X3: Cholesterol	1038.79	< 0.0001	448.68	< 0.0001	2.11	0.1716	372.50	< 0.0001
X32	42.28	< 0.0001	25.22	0.0004
Lack of Fit	1.58	0.3854	0.8009	0.6452	1.59	0.3853	0.4333	0.8549

Model	Reduced Quadratic	Reduced Quadratic	Linear	Linear
Adjusted R2	0.9872	0.9747	0.8866	0.9649
R2	0.9906	0.9815	0.9093	0.9719
%CV	3.33	3.27	6.50	1.01
Predicted R2	0.9783	0.9600	0.8366	0.9526
Adequate precision	51.5296	39.5747	17.4094	36.5268
Standard deviation	6.37	1.71	1.87	0.0869

$\mathrm{VS}=180.7+6.05.X_1+19.15.X_2+72.55.X_3+20.7.X_3^2$

$\mathrm{EE}=54.4+6.1.X_1+1.5.X_2+12.8.X_3-4.3.X_3^2$

$\mathrm{ZP}=28.8-0.35.X_1+7.19.X_2-0.96.X_3$

$\text{Sqrt}\left(\mathrm{CR}\right)=8.6-0.2.X_1-0.016.X_2-0.59.X_3$