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Recovery of MAO-B inhibition by IHC3 using dialysis experiments. The concentrations of safinamide (reversible MAO-B inhibitor), pargyline (an irreversible MAO-B inhibitor), and IHC3 used were ~2.0 times their IC50 values (0.04, 0.28, and 3.20 μM, respectively). After preincubation for 30 min, the mixtures were dialyzed for 6 h, with two buffer changes.
