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. 2023 Aug 16;15(16):3596. doi: 10.3390/nu15163596

Figure 5.

Figure 5

The inhibitory effect of gallic acid on the invasion of MCF7-6.4/12w cells is related to the inhibition of the PI3K/Akt signaling-controlled β-catenin pathway. (A–D) Western blot assay was used to analyze total and nuclear β-catenin levels in MCF7 and MCF7-6.4/12w cells (A) and to assess the expression levels of p85, pAKT, and total AKT (C). In MCF7-6.4/12w cells, after treatment with 0, 10, and 20 μM of gallic acid, total and nuclear β-catenin levels (B), along with the expression levels of p85, pAKT, and total AKT (D), were also evaluated using a Western blot assay. (E,F) An invasion assay was conducted using normal MCF7 cells and MCF7-6.4/12w cells treated with either 1 μM LY294002, 1 μM AKT Inhibitor VIII, or untreated (E), and the relative ratio of invaded cells compared to normal MCF7 cells is represented (F). (G) MCF7-6.4/12w cells treated with 1 μM LY294002, 1 μM AKT Inhibitor VIII, or untreated, were analyzed by Western blot assay to detect total and nuclear β-catenin levels. Lamin B is nuclear loading control. * p < 0.05 vs. MCF7, # p < 0.05 vs. untreated MCF7-6.4/12w. Scale bar = 100 μm.