Table 2.
Physicochemical characterization of mRNA LNPs in application buffer (10 mM glycylglycine, pH 5.7). Data presented as mean ± S.D., n = 3.
| Formulation | Composition | Diameter (nm) | PDI | Zeta Potential (mV) |
Inaccessible mRNA (%) |
|---|---|---|---|---|---|
| LNP 1 | DODMA/DOPE/PEG | 201 ± 15 | 0.202 ± 0.043 | 27 ± 7 | 95 ± 3 |
| LNP 2 | DODMA/DOPE/pSar | 239 ± 21 | 0.173 ± 0.028 | 34 ± 6 | 91 ± 4 |
| LNP 3 | DODMA/DOPC/PEG | 221 ± 15 | 0.205 ± 0.021 | 28 ± 5 | 92 ± 4 |
| LNP 4 | DODMA/DOPC/pSar | 242 ± 33 | 0.193 ± 0.040 | 35 ± 3 | 90 ± 4 |
| LNP 5 | MC3/DOPE/PEG | 174 ± 07 | 0.124 ± 0.021 | 30 ± 3 | 96 ± 2 |
| LNP 6 | MC3/DOPE/pSar | 196 ±13 | 0.170 ± 0.015 | 33 ± 2 | 93 ± 2 |
| LNP 7 | MC3/DOPC/PEG | 159 ± 15 | 0.117 ±.0.024 | 27 ± 6 | 97 ± 1 |
| LNP 8 | MC3/DOPC/pSar | 197 ± 17 | 0.156 ± 0.028 | 34 ± 1 | 94 ± 2 |