Table 1.

Primer sequence used for real-time PCR.

Gene	Forward Primer	Reverse Primer	bp	Accession No.	References
TGF-β1	AGGGCTACCATGCCAACTTC	CCACGTAGTAGACGATGGGC	168	NM_021578.2	[40]
Smad-7	GAGTCTCGGAGGAAGAGGCT	CTGCTCGCATAAGCTGCTGG	84	NM_030858.2	[40]
Smad-3	CTGGGCAAGTTCTCCAGAGTT	AAGGGCAGGATGGACGACAT	148	NM_013095.3	[40]
Beclin-1	GAATGGAGGGGTCTAAGGCG	CTTCCTCCTGGCTCTCTCT	180	NM_001034117.1	[40]
LC-3	GAAATGGTCACCCCACGAGT	ACACAGTTTTCCCATGCCCA	147	NM_012823.2	[40]
mTOR	GCAATGGGCACGAGTTTGTT	AGTGTGTTCACCAGGCCAAA	94	NM_019906.2	[40]
P62	GGAAGCTGAAACATGGGCAC	CCAAGGGTCCACCTGAACAA	183	NM_181550.2	[40]
SNHG-7	TGGCAGTGTCTTAGCTGGTT	AACGTGCAGCACTTCTAGGG	81	NR_031850.1
Coli-1	GCAATGCTGAATCGTCCCAC	CAGCACAGGCCCTCAAAAAC	176	NM_053304.1	[41]
AMPK	GCGTGTGAAGATCGGACACT	TGCCACTTTATGGCCTGTCA	103	NM_023991.1
AKT-1	GAAGGAGAAGGCCACAGGTC	TTCTGCAGGACACGGTTCTC	111	NM_033230.3
ULK-1	CGTACACTGCCTGACCTCTC	AGAGGCCTGTGTCCCAAATG	162	NM_001108341.1
rat Gapdh	GGCACAGTCAAGGCTGAGAATG	ATGGTGGTGAAGACGCCAGTA	143	NM_017008.4	[35]
human Gapdh	GGAGTCAACGGATTTGGTCGT	ACGGTGCCATGGAATTTGC	161	NM_002046.7	[35]
mir-29b	AACACGCCTGGTTTCACATG	GTCGTATCCAGTGCAGGGT
mir-34a	AACACGCTGGCAGTGTCTTA	GTCGTATCCAGTGCAGGGT
mir-181	AACACGCAACATTCAACGCT	GTCGTATCCAGTGCAGGGT
Let-7b	AACACGCTGAGGTAGTAGGTT	GTCGTATCCAGTGCAGGGT			[40]
U6	GCTCGCTTCGGCAGCACA	GAGGTATTCGCACCAGAGGA			[40]
mir-29b stem-Loop primer		GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTAAGC
mir-34a stem-Loop primer		GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAACC
mir-181 stem-Loop primer		GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTCAC
Let-7b stem-Loop primer		GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACCAC			[40]
U6 stem-Loop primer		AACGCTTCACGAATTTGCGTG			[40]