Table 1.
The Ct values obtained from the two methodologies were compared to confirm the lack of amplification of the N gene. The samples tested with the Allplex SARS-CoV-2 Extraction-Free Assay were confirmed positive with the Xpert Xpress SARS-CoV-2 Assay through which the amplification of the N2 target was present.
| Sample Number | Variant | Allplex SARS-CoV-2 Extraction-Free Assay | Xpert Xpress SARS-CoV-2 Assay | |||||
|---|---|---|---|---|---|---|---|---|
| E Gene | RdRP/S Gene | N Gene | IC * | E Gene | N2 Gene | IC * | ||
| 1 | B.1.1.7 | 25.98 | 29.02 | N/A | 22.05 | 20.9 | 24.1 | 27.6 |
| 2 | B.1.1.7 | 21.23 | 22.34 | N/A | 22.45 | 17.1 | 17.9 | 27.5 |
| 3 | B.1.1.7 | 19.18 | 21.25 | N/A | 22.12 | 14.9 | 16.3 | 27.5 |
| 4 | B.1.1.7 | 19.12 | 22.01 | N/A | 21.98 | 15.8 | 17.1 | 27.1 |
| 5 | B.1.1.7 | 24.45 | 26.68 | N/A | 22.01 | 20 | 21.7 | 27.2 |
| 6 | B.1.1.7 | 19.04 | 21.32 | N/A | 21.77 | 15 | 16.6 | 28.3 |
| 7 | B.1.1.7 | 23.12 | 24.23 | N/A | 22.13 | 18.6 | 19.3 | 27.4 |
| 8 | B.1.1.7 | 25.98 | 28.1 | N/A | 22.31 | 21.8 | 23.4 | 27.7 |
| 9 | B.1.1.7 | 27.98 | 30.01 | N/A | 21.89 | 22.9 | 25.4 | 28.1 |
| 10 | B.1.1.7 | 26.78 | 29.07 | N/A | 22.52 | 21.8 | 24.7 | 28.5 |
| 11 | B.1.1.7 | 24.98 | 28.13 | N/A | 22.33 | 20.7 | 23.3 | 28.2 |
| 12 | B.1.1.7 | 23.37 | 23.98 | N/A | 21.88 | 19.7 | 19.8 | 27.3 |
| 13 | B.1.1.7 | 23.01 | 26.25 | N/A | 21.91 | 18.5 | 21.4 | 27.1 |
| 14 | B.1.1.7 | 24.55 | 27.32 | N/A | 22.12 | 20.2 | 23.1 | 28.3 |
| 15 | B.1.1.7 | 23.72 | 26.03 | N/A | 22.37 | 18.7 | 21.3 | 27.5 |
| 16 | B.1.1.7 | 20.34 | 22.22 | N/A | 22.41 | 16.12 | 17.6 | 28.0 |
| 17 | B.1.1.7 | 21.23 | 24.37 | N/A | 22.4 | 17.06 | 19.5 | 28.1 |
| 18 | B.1.1.7 | 18.11 | 21.01 | N/A | 21.83 | 14.94 | 16.3 | 27.9 |
| 19 | B.1.1.7 | 19.23 | 19.99 | N/A | 21.85 | 14.78 | 15.3 | 27.4 |
| 20 | B.1.1.7 | 22.12 | 23.92 | N/A | 22.01 | 17.63 | 19.5 | 28.0 |
| 21 | B.1.1.7 | 19.15 | 22.32 | N/A | 21.99 | 15.06 | 17.8 | 28.4 |
| 22 | B.1.1.7 | 23.04 | 24.59 | N/A | 22.38 | 19.06 | 20.9 | 27.8 |
| 23 | B.1.1.7 | 24.46 | 27.12 | N/A | 22.52 | 20.1 | 23.0 | 27.1 |
| 24 | B.1.1.7 | 20.67 | 21.89 | N/A | 22.04 | 16.4 | 17.7 | 27.3 |
| 25 | B.1.1.7 | 28.47 | 30.15 | N/A | 22.56 | 24.1 | 25.6 | 28.2 |
| 26 | B.1.1.7 | 25.34 | 29.07 | N/A | 22.63 | 21.1 | 24.3 | 28.5 |
| 27 | B.1.1.7 | 23.34 | 25.87 | N/A | 22.43 | 19.5 | 22.1 | 27.3 |
| 28 | B.1.1.7 | 26.76 | 29.1 | N/A | 22.76 | 22.3 | 24.8 | 27.8 |
| 29 | B.1.1.7 | 23.53 | 26.67 | N/A | 22.51 | 19.7 | 22.4 | 27.3 |
| 30 | B.1.1.7 | 24.79 | 27.2 | N/A | 22.49 | 21.1 | 23.0 | 28.1 |
| 31 | B.1.1.7 | 19.55 | 21.68 | N/A | 21.81 | 15.2 | 17.5 | 27.7 |
| 32 | AY.102 | 22.13 | 23.54 | N/A | 22.06 | 18.1 | 19.1 | 27.4 |
| 33 | B.1.617.2 | 17.77 | 21.98 | N/A | 21.86 | 14.8 | 18.2 | 28.5 |
| 34 | AY.43 | 23.51 | 25.12 | N/A | 22.32 | 19.6 | 21.0 | 28.5 |
| 35 | AY.43 | 21.88 | 24.56 | N/A | 22.44 | 18.2 | 20.2 | 27.0 |
| 36 | AY.43 | 25.34 | 27.89 | N/A | 22.61 | 21.8 | 24.1 | 27.4 |
| 37 | AY.43 | 18.87 | 21.03 | N/A | 21.93 | 15 | 16.6 | 28.6 |
| 38 | AY.23 | 18.56 | 20.45 | N/A | 22.14 | 15.2 | 17.0 | 27.9 |
| 39 | AY.23 | 23.34 | 25.12 | N/A | 22.25 | 19.3 | 20.6 | 28.6 |
| 40 | AY.23 | 20.06 | 22.76 | N/A | 22.17 | 16 | 18.2 | 27.2 |
| 41 | AY.23 | 26.34 | 28.98 | N/A | 22.78 | 22.1 | 25.0 | 27.6 |
| 42 | BA.1.1 | 17.88 | 19.34 | N/A | 22.21 | 14.4 | 15.4 | 28.1 |
| 43 | BA.1 | 20.91 | 22.65 | N/A | 22.01 | 16.5 | 18.4 | 27.3 |
| 44 | BA.1.21 | 20.67 | 21.76 | N/A | 22.32 | 17.1 | 18.2 | 27.5 |
| 45 | BA.2 | 17.76 | 18.63 | N/A | 21.97 | 14.2 | 14.0 | 27.2 |
| 46 | BA.2.36 | 20.09 | 22.87 | N/A | 22.31 | 16.1 | 18.0 | 28.0 |
| 47 | BA.5 | 29.03 | 29.77 | N/A | 22.4 | 24.7 | 25.7 | 28.3 |
| 48 | BA.5.1 | 18.36 | 20.87 | N/A | 22.35 | 15.1 | 16.0 | 27.9 |
* IC: internal control.