FIG. 3.

Multiplex PCR analysis of primary fecal cultures. Crude DNA extracts of primary fecal cultures were analyzed by multiplex PCR assay 1 or assay 2, as indicated. Lanes: 1, negative control; 2, patient 1 (HUS); 3, patient 2 (HUS); 4, patient 3 (HUS); 5, patient 4 (HUS); 6, patient 5 (bloody diarrhea); 7, patient 6 (control); 8, patient 7 (control). The expected mobilities for the various specific PCR products are also indicated.