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. 1998 Feb;36(2):598–602. doi: 10.1128/jcm.36.2.598-602.1998

FIG. 4.

FIG. 4

Sensitivity of multiplex PCR assay 2. An STEC-negative fecal culture was spiked with serial 10-fold dilutions of cultures of STEC strains 95NR1 (O111:H−) or 95SF2 (O157:H−). Extracts of these samples were then subjected to assay 2. Lanes: M, DNA size markers (pUC19 DNA digested with HpaII; fragment sizes visible are 501/489, 404, 331, 242, 190, 147, and 111 bp); 1, negative control; 2, STEC-negative fecal culture (FC); 3, FC plus a 10-fold dilution of a strain 95SF2 culture (10−1 95SF2); 4, FC plus 10−2 95SF2; 5, FC plus 10−3 95SF2; 6, FC plus 10−4 95SF2; 7, FC plus 10−5 95SF2; 8, FC plus 10−1 95NR1; 9, FC plus 10−2 95NR1; 10, FC plus 10−3 95NR1; 11, FC plus 10−4 95NR1; 12, FC plus 10−5 95NR1; 13, FC plus 10−1 95SF2 plus 10−1 95NR1. The expected mobilities for the O111- and O157-specific PCR products are also indicated.