Fig. 5.

Overexpression of DsbA-L alleviated leakage of peroxisomal membrane, proteasomal degradation of peroxisomal matrix proteins, peroxisomal H₂O₂and apoptosis in HK-2 cells induced by HG and PA. (A) Cells were treated with NG, HG and PA, or MG132 (10 μM) for 24 h and followed by immunoblotting. (B) HK-2 Cells were treated with NG, HG and PA, or DsbA-L plasmid, followed by Proteinase K protection assay, and immunoblotting for PMP70, CAT and ACOX1. (C) HK-2 cells were treated with DCFH-DA to determine the ROS and examined under fluorescence microscope. (D) Peroxisomal H₂O₂ production was measured by HyPer-PTS1(n=3). (E) Representative western blot bands of Caspase-9, BCL2, BAX and Cleaved Caspase-3 protein in HK-2 cells. (F) Relative band intensity of Caspase-9, BCL2, BAX and Cleaved Caspase-3 protein in HK-2 cells (n=3). Data are presented as means±SEMs. *P < 0.05vs NG; **P < 0.05vs HG and PA; ns, not significant.