Fig. 6.

Peroxisomal localization of DsbA-L improved peroxisomal function and apoptosis. (A) Construction of DsbA-L plasmid directed to peroxisome. (B) The DsbA-L knockout stable transfection strain (DsbA-L-KO) was constructed, and DsbA-L-PTS1 was transfected in the DsbA-L-KO stable transfection strain. Panel shows the DsbA-L as red fluorescence, the peroxisomes showed as PMP-70 positive green staining, and the co-localization of DsbA-L with PMP-70 is shown as yellow fluorescence puncta. Nuclear DNA is stained with DAPI and shown in blue. Scale bar: 20 μm. (C) Peroxisomal H₂O₂ production was measured by HyPer-PTS1 (n=3). (D) Representative western blot bands of PMP70, CAT, DsbA-L, BCL2, BAX and Cleaved Caspase-3 protein in HK-2 cells. (E) Relative band intensity of PMP70, CAT, BCL2, BAX and Cleaved Caspase-3 protein in HK-2 cells (n=3). Data are presented as means ± SEMs. *P < 0.05vs NG; **P < 0.05vs HG and PA; ns, not significant. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)