Figure 3.

Open field test. On the 8th and 16th days after the last injection, the animals were placed in the center of an open field and left for 10 min to explore it. The total distance covered across time (cm) (A,E) showed that all the groups successfully habituated to the task. The total distance covered (cm) (B,F), percentage of distance at the center of the box (C,G), and time at the center of the box (s) (D,H) were calculated using the MouseGlob v. 1.0 free software. No treatment effect was observed in terms of the mice’s locomotion and thigmotaxis. The analyses were performed via two-way ANOVA for repeated measures (A,E) or one-way ANOVA (B–D,F–H), followed by Tukey’s post hoc test. The results are expressed as the mean ± SEM (n = 15).