Expression of TNF-α and IL-1β in Cartilage Tissue with ACLT after Treatment. (A,I) indicate control group; (B,J) indicate sham group; (C,K) indicate ACLT group; (D,L) indicate group A; (E,M) indicate group B; (F,N) indicate group C; and (G,O) indicate group D. Brown areas indicate immunoreactive cells. (A–G) Immunohistochemical staining of TNF-α in cartilage. (H) Quantitative analysis of TNF-α–positive cells. (I–P) Immunohistochemical staining of IL-1β in cartilage. (P) Quantitative analysis of TNF-α–positive cells. Scale bar = 100 μm. Data are presented as mean ± standard error of the mean. * denotes comparison with sham group (p < 0.05); # denotes comparison with ACLT group (p < 0.05).