FIG. 2.

Ubiquity test by dot blot hybridization by using the ³²P-labeled, S. aureus-specific, 442-bp DNA fragment as a probe and DNAs from 82 clinical isolates of S. aureus as targets. DNAs from the S. aureus strains are at locations 1A (ATCC 43300), 12A (ATCC 25923), and 1B to 8H (80 clinical strains from CHUL). A battery of eight different staphylococcal species including S. epidermidis, S. saprophyticus, S. simulans, S. lugdunensis, S. haemolyticus, S. hominis, S. warneri, and S. schleiferi were used as negative controls (locations 3A to 10A, respectively). Micrococcus luteus was also used as a negative control (location 11A). DNA from transformed E. coli DH5α carrying the recombinant plasmid pGEM-Sa442 was spotted at location 2A. No DNA was spotted at locations 9H to 12H.