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Competitive RT-PCR assay. (a) A constant amount of st2 RNA was coamplified with increasing amounts of wt1 RNA, followed by hybridization with an st2-specific capture probe. (b) A constant amount of wt1 RNA was coamplified with increasing amounts of st2 RNA, followed by hybridization with a wt1-specific capture probe. No competition could be seen in either case over a 5-log dynamic range.
