Figure 8.

Evaluation of calix[4]arene vectors’ efficiency for the delivery of premiRNA molecules. (A,B) Quantification of miR-221-3p intracellular content within U251 cells after the transfection of premiR-221-3p delivered by calix[4]arene vectors. For each vector the fold change was calculated with respect to cells treated with the vector, not complexed with premiRNA. (C,D) Quantification of miR-221-3p intracellular content within HT29 cells after the transfection of premiR-221-3p carried by calix[4]arene vectors. Quantification of miR-221-3p has been performed by RT-qPCR. Fold change with respect to the cells treated with only the vector was calculated. Results are presented as mean ± S.E.M; statistical differences between groups were compared using a one-way repeated-measures ANOVA. * p < 0.05, ** p < 0.01.