Table 1.

Primers used to target the 16S rRNA subregion.

Region	Forward	Reverse	Size (bp)
V1–V2	AGAGTTTGATYMTGGCTCAG	TGCTGCCTCCCGTAGRAGT	311
V1–V3	TNANACATGCAAGTCGRRCG	WTTACCGCGGCTGCTGG	450
V3–V4	CCTACGGGNGGCWGCAG	GACTACHVGGGTATCTAATCC	444
V4–V5	GTGYCAGCMGCCGCGGTAA	CCGYCAATTYMTTTRAGTTT	411
V5–V7	AACMGGATTAGATACCCKG	ACGTCATCCCCACCTTCC	394
V6–V8	CAACGCGAAGAACCTTACC	GACGGGCGGTGWGTRCA	424