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. 2023 Aug 22;17:2461–2479. doi: 10.2147/DDDT.S414098

Figure 3.

Figure 3

In vitro pro-apoptotic effects of QBD (0, 1.5, 2.25, and 3 mg/mL) on H1299 cells. (A) Morphological observation of H1299 cells treated with QBD (0, 1.5, 2.25, and 3 mg/mL) or DDP (22.5 μg/mL) for 24 h. (B) Cell viability of H1299 cells treated with QBD (0, 1, 1.5, 2, 3, and 4 mg/mL) for 24 and 48 h. (C) DAPI staining, TUNEL assay observations of H1299 cells after 24 h treatment of QBD (0, 1.5, 2.25, and 3 mg/mL) or DDP (22.5 μg/mL), with white arrows indicating TUNEL positive cells. (D) Flow cytometry analysis of H1299 cells after 24 h treatment of QBD (0, 1.5, 2.25, and 3 mg/mL) or DDP (22.5 μg/mL) and the statistical analyses of the apoptotic rate. LL: FITC-Annexin V and PI negative; LR: FITC-Annexin V positive; UR: FITC-Annexin V and PI positive; UL: PI positive, Values were presented as the mean ± SD, *P < 0.05, **P < 0.01, and ***P < 0.001 vs control group. Scale bar: 50 μm.