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. 2023 Aug 26;14(8):566. doi: 10.1038/s41419-023-06091-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A USP14 and CYP2E1 protein expression in AML12 cells treated with MG132 and CQ and transfected with the corresponding vectors. B Co-IP assay to assess ubiquitination of HSP90AA1 in HEK-293T cells transfected with the corresponding labeled USP14, HSP90AA1, or ub plasmids. C Construction of an active site mutant of USP14 (USP14 C114A) and a truncation mutant of USP14 lacking the UBL. D Co-IP analysis of the interaction domain of USP14 and HAP90AA1. E Co-IP analysis of the interaction domain of USP14 on HAP90AA1 deubiquitination. F Identification of the type of HAP90AA1 deubiquitination by USP14. G Analysis of protein levels of CYP2E1 after AML12 cells were transfected with Ad-CMV, Flag-USP14 FL, Flag-C114A, and Flag-∆UBL. **P < 0.01 between the two groups. NS not significant.